Journal: Pharmaceuticals
Article Title: Exploring the Anti-Osteoporotic Effects of n -Hexane Fraction from Cotoneaster wilsonii Nakai: Activation of Runx2 and Osteoblast Differentiation In Vivo
doi: 10.3390/ph18010045
Figure Lengend Snippet: Effects of of n -haxane fraction of C. wilsonii (CWH) on physiological markers in ovariectomized mice. ( A ) Experimental design for animal study. ( B ) Measurements of body weight, uterus weight, and serum osteocalcin levels in OVX mice treated with CWH. ( C ) Histological analysis using H&E staining and type I collagen (COL1) immunohistochemical staining in the distal femoral region of OVX mice. Data are presented as mean ± SEM (n = 6). * p < 0.05, ** p < 0.01, and *** p < 0.001 are significantly different from the OVX group. Blue arrows: lipid droplets; Red arrows: collagen.
Article Snippet: Serum osteocalcin (OCN) levels were quantified using the Gla-type osteocalcin (Gla-OC) EIA Kit (TaKaRa, Tokyo, Japan).
Techniques: Staining, Immunohistochemical staining